I mea e loiloi ai i ka hopena o ka hoʻohui ʻana i ka triglyceride i ka meaʻai ma ka hana ʻana o ka protein microbial rumen a me nā ʻano fermentation o nā hipa wahine huelo liʻiliʻi makua, ua hana ʻia ʻelua mau hoʻokolohua in vitro a me in vivo.

Hoʻāʻo in vitro: ua hoʻohana ʻia ka ʻai basal (ma muli o ka mea maloʻo) me nā pae triglyceride o 0, 2, 4, 6 a me 8g / kg ma ke ʻano he substrate, a ua hoʻohui ʻia ka wai rumen o nā hipa wahine Adult Small Taled, a ua hoʻomoʻa ʻia ma 39 ℃ no 48h in vitro.

Hoʻāʻo in vivo: Ua puʻunaue ʻia he 45 mau hipa wahine makua i 5 mau pūʻulu e like me ko lākou kaumaha mua (55 ± 5 kg).ʻO ka hui Glycerylua hoʻohui ʻia o 0, 2, 4, 6 a me 8 g/kg (ma muli o ka mea maloʻo) i ka ʻai maʻamau, a ua hōʻiliʻili ʻia ka wai rumen a me ka mimi no 18 mau lā.

Hopena Hoʻāʻo

1). Hopena ma ka waiwai pH a me ka nui o ka waikawa momona volatile

Ua hōʻike ʻia nā hopena ua emi iho ka waiwai pH o ka medium culture a ua piʻi aʻe ka nui o nā ʻāpana o ka volatile fatty acids (TVFA), acetic acid, butyric acid a me nā branched chain volatile fatty acids (BCVFA) i ka wā i hoʻonui ʻia ai.ʻO ka tributyl glycerideua hoʻohui ʻia i ka substrate. Ua hōʻike nā hopena o ka hoʻāʻo in vivo ua emi ka lawe ʻana o ka mea maloʻo (DMI) a me ka waiwai pH, a ua hoʻonui ʻia nā ʻano o TVFA, acetic acid, propionic acid, butyric acid a me BCVFA me ka hoʻohui ʻana oʻO ka tributyl glycerideUa hōʻike ʻia nā hopena o ka hoʻāʻo in vivo ua emi ka lawe ʻana o ka mea maloʻo (DMI) a me ka waiwai pH, a ua piʻi pololei nā ʻano o TVFA, acetic acid, propionic acid, butyric acid a me BCVFA me ka hoʻohui ʻana o tributyl glyceride.

Ua hōʻike ʻia nā hopena o ka hoʻāʻo in vivo ua emi ka lawe ʻana o ka mea maloʻo (DMI) a me ka waiwai pH, a ua piʻi pololei nā ʻano o TVFA, acetic acid, propionic acid, butyric acid a me BCVFA me ka hoʻohui ʻana oʻO ka tributyl glyceride.

2). Hoʻomaikaʻi i ka helu hōʻino o nā meaʻai

Ua piʻi pololei ka nui o ka hōʻino ʻia ʻana o DM, CP, NDF a me ADF i ka wāʻO ka tributyl glycerideua hoʻohui ʻia i loko o ka substrate in vitro.

3). Hoʻomaikaʻi i ka hana o ka enzyme cellulose degrading

ʻO ka hoʻohui ʻana oʻauhauin vitro ua hoʻonui linear ʻia nā hana o ka xylanase, carboxymethyl cellulase a me ka microcrystalline cellulase. Ua hōʻike nā hoʻokolohua in vivo ua hoʻonui linear ʻia nā hana o ka triglyceride o ka xylanase a me ka carboxymethyl cellulase.

4). Hoʻomaikaʻi i ka hana ʻana o ka protein microbial

Ua hōʻike ʻia nā hoʻokolohua in vivoʻauhauua hoʻonui linearly i ka nui o kēlā me kēia lā o ka allantoin, uric acid a me ka purine microbial i hoʻopili ʻia i loko o ka mimi, a ua hoʻonui i ka synthesis o ka rumen microbial nitrogen.

Hopena

ʻO Tributyrinua hoʻomaikaʻi i ka synthesis o ka protein microbial rumen, ka ʻike o nā waikawa momona volatile a me ka hana o nā enzymes cellulose degrading, a ua hoʻolaha i ka degradation a me ka hoʻohana ʻana i ka mea maloʻo, ka protein maka, ka fiber detergent neutral a me ka fiber detergent acid i ka meaʻai.

Hōʻike ia he hopena maikaʻi ko ka triglyceride i ka hua a me ka fermentation o ka protein microbial rumen, a he hopena maikaʻi paha ia i ka hana hana o nā hipa wahine makua.